The heel stick method of capillary blood sampling will be described, but capillary blood samples can also be obtained from a finger, toe, or earlobe. Blood sampling from laboratory animals is a routine procedure in biomedical research. Adults blood sampling in amounts of up to 200 cc, drawn at one time o in general, blood sampling in amounts totaling up to 200 cc may be removed from a volunteer subject that upon examination appears healthy, without further precaution. Venepuncture is the preferred method of blood sampling in infants, as it causes less pain, offers more reliable collection, and therefore less need for repeat sampling. Blood clotting time determination by capillary method. Genieur standard operating procedure sop blood sampling and transportation 5. The purpose of this standard operation procedure is to ensure that the process of blood sample collection for nutrition centres is performed under standardised conditions. Explain the procedure and reason for the blood draw to the patient. Closed blood sampling vamp system edwards lifesciences. Research nurse practitioners clinical research fellows members of clinical staff trained to take blood, including doctors and nurses on the unit. Download 1,209 nurse taking blood sample stock photos for free or amazingly low rates.
Hence, accurate quantitative data can only be obtained if the size of the paper filter extracted contains a fixed blood volume. In probability sampling, each unit is drawn with known probability, yamane, p3 or has a nonzero chance of being selected in the sample. These are usually collected from an appropriate vein, but arterial samples are required for some special techniques. Refer to your facility safety manual for further information on the recapping of needles. Therefore, composite sampling is normally described as either flow based or time based. This document covers all steps of the venous blood collection procedure for in and outpatients. However, in the mouse, which is the laboratory animal used by far the most in the european union, 1 serial blood sampling is complicated by the limited blood volume of the animal as well as by animal welfare concerns and practical difficulties related to puncturing available venous access sites repeatedly. Check out this real time video being performed on real patient. Specimen collection methods include venipuncture, where blood is collected by. Knowledge of good blood culture sampling practice among.
Collecting, processing,and handling venous, capillary, and blood. Preanalytical errors related to venous sample collection and. Although it can be difficult to collect blood from the small veins of exotic mammals, one can take some consolation in knowing that there are similarities in the vascular anatomy between these animals and larger domestic species that allow for similar approaches for collecting blood samples. Collection of blood samples rat, mouse, guinea pig, rabbit. A barrier material, such as silicone that is sprayed on the finger at this point in the process, will help a distinct bead of. Sampling design and procedures stratified sampling. Neonatal pain response to heel stick vs venepuncture for.
The vamp adult system is designed to reduce infection and blood waste associated with traditional blood sampling. Approved ifcc recommendations on whole blood sampling, transport and storage for simultaneous determination of ph, blood gases and electrolytes. Arterial catheter blood samplingblood pressure monitoringcatheter removal cc 80005 page 3 of 20 learning module this is a controlled document for internal use only. Prior to the analysis of a blood sample, multiple steps are necessary to. The use of personal protective equipment ppe is therefore mandatory if direct close contact with. Always wear gloves, throughout the entire procedure, when obtaining blood specimens. Pdf a procedure for arterial blood sampling in the rabbit. Collection of blood samples rat, mouse, guinea pig, rabbit introduction many research programmes require the collection of blood samples from animals. Target audience for this recommendation are healthcare staff members. Food sampling guidelines and procedures providing samples at farmers markets allows consumers to try a product before purchasing it. Learn the methods to humanely rem ove blood samples from anaesthetized fish. The phlebotomist will insert a needle into a vein in their arm similar to giving a blood sample in a lab that may be requested by their family physiciandoctor during a routine physical or a checkup. Blood collection techniques in exotic small mammals.
Used to choose the number of participants, interviews, or work samples to use in the assessment process. Scientific division committee on ph, blood gases and electrolytes. The guinea pig is a valuable and validated experimental animal model due to a number of biological similarities to humans such as a requirement for a dietary supply of vitamin c, comparable plasma lipoprotein metabolizing enzymes and lipoprotein profiles, as well as shared similarities with human placentation and prenatal development,7,8. The sampling was finished within one minute and the amount of blood sampled was about 30. Sampling is a procedure, where in a fraction of the data is taken from a large set of data, and the inference drawn from the sample is extended to whole group. Apply a tourniquet proximal to the site of venepuncture to ensure engorgement of vein with blood. Jamil ahmad khan head of sample management department. Laboratory sample tubes for blood collection sterile glass or plastic tubes with rubber caps, vacuumextraction blood tubes, or glass tubes with screw caps. Percutaneous blood sampling article about percutaneous. How to safely collect blood samples from persons suspected to be infected with highly infectious bloodborne pathogens e. Used needles and syringes or blood sampling devices must be discarded into a punctureresistant sharps container. Nonterminal blood sampling techniques in guinea pigs.
A n arterial blood gas abg test measures the amounts of arterial gases, such as oxygen and carbon dioxide. International federation of clinical chemistry ifcc. In this sense, the sample is the gathered matter, and the sampling tool or sampler is the person or material to collect the sample sampling is a prerequisite for many medical tests, but generally. Spot sampling, by definition, is different from composite sampling or constant sampling. Blood sample definition of blood sample by medical dictionary. Veins are large, thinwalled blood vessels which carry blood back to the heart.
Prepare a 10ml syringe with either a green or blue needle depending upon the size of the vein or prepare the vacutainer with a butterfly needle. Hemodraw is smiths medical s needle free closed blood sampling system for arterial blood sampling in adults. Identify the patient using two patient identifiers as mandated by jcaho. The first drop of blood contains tissue fluids that will produce inaccurate results. The blood can be taken from a vein where it is called venous blood collection or arteries where it is called arterial blood collection. Article pdf available in methods in molecular biology 428. For use with disposable pressure transducers and for connection to central line catheters and arterial. If an incident that could lead to infection occurs during a sampling procedure such as a breakdown of protective procedures the staff members involved should be monitored. How to safely collect blood samples from persons suspected to. In medicine, sampling is gathering of matter from the body to aid in the process of a medical diagnosis andor evaluation of an indication for treatment, further medical tests or other procedures. Combination intravenous infusion blood sampling and medication injection apparatus is provided whereby a patient may be administered an intravenous infusion fluid and be subjected to blood sampling or medication injector procedures without the need to make or break any fluid connection in a sealed closed system in order to prevent possible contamination from entering the apparatus and causing. Pain was assessed by nurses using the neonatal infant pain scale nips and a three point scale for the mothers. Blood sampling or blood collection method is an essential procedure in modern medicine. Since farmers markets sell primarily on taste, sampling is a top marketing tool.
Nurse taking blood sample stock photos download 1,209. Blood or other samples are taken at bedside from patients by a technician, nurse, or the like who carries a microprocessoroperated device which optically scans the coded patient identification bracelet. Who library cataloguinginpublication data who guidelines on drawing blood. To facilitate blood flow, many procedures call for the collection container to be held nearly horizontal, with a slight downward angle. The unique design makes hemodraw safe, accurate and easy to handle in both operating rooms and intensive care units. Pdf do blood sampling sites affect pharmacokinetics. Because hct determines the viscosity of blood, it may affect the spreading of blood onto the filter paper. A limited number of test procedures can be done on micro blood samples. Prepare a 10ml syringe with either a green or blue needle depending upon the size. Multidraw needles for use with evacuated collection tubes. Hemodraw is a unique, needle free closed blood sampling system which protects both clinician and patient. Capillary blood sampling can be used to test for, for example, blood glucose such as in blood glucose monitoring, hemoglobin, ph and lactate. Full blood count, urea and electrolytes, drug testing appropriately trained staff can perform a venepuncture. Division of academic and student affairs office of assessment sampling procedure 1 of 2 sampling procedure definition sample.
Arterial blood specimen is the first choice for bga for assessing ventilation and acid base status. Pdf blood sample collection and patient identification demand. This makes the guinea pig an attractive and suitable. As you can see in this diagram, there are many veins in this area that are close to the surface of the skin. Scribd is the worlds largest social reading and publishing site. Grasp the patients arm or hand with your free hand to hold the skin and. The material in this manual may be freely used for educational or. How to safely collect blood samples from persons suspected. The end of the tail was cut by razor blade and a few drops of blood were collected into a heparinized capillary tube. Blood sampling systems needle and syringe system, vacuum extraction system with holder, winged butterfly system vacuum extraction or winged butterfly system syringe tourniquet singleuse skin antiseptic solution. This paper is the results of a pilot project that we conducted in developing our structured learning package for good blood culture.
Hematocrit hct is one of the most critical issues associated with the bioanalytical methods used for dried blood spot dbs sample analysis. The steps in the procedure are based on the best available evidence and a consensus opinion was reached following detailed. Probability sampling a term due to deming, deming is a sampling porcess that utilizes some form of random selection. Us4865583a combination blood sampling and intravenous. Blood samples were obtained from individual pups by a serial sampling method 1. The site of collection varies with the species and the circumstances. The effect of this on the animals concerned may be minimal or profound, depending on the amount collected, the technique used, the frequency of sampling and the skill of the operator. New microfluidicbased sampling procedure for overcoming. However, arterial blood collection causes patient discomfort and other complications. The most commonly used vein for venous blood sampling is found in the antecubital fossa, that is the area inside the bend of the elbow figure 7a. An abg test measures the bloodgas tension values of the arterial partial pressure of oxygen, and the arterial partial pressure of carbon dioxide, and the.
Safety considerations a white laboratory coat or tunic is worn and fastened to the top. Sampling design and procedures free download as powerpoint presentation. This is especially important when the farmer is offering a new or different product that the customer has never tried before. How to perform sampling and collect the blood from radial, femoral or brachial artery for abg tests such as ph, carbon dioxide and oxygen saturation, etc. Obtain assistance for placement of blood into containers if this will take more than one minute because stationary blood in the line may result in clot formation. Jamil ahmad khan head of sample management department, talking about blood sample collection. Remove syringe from the sampling site by pulling straight out. Specimens may be stored in glass or plastic vials, as long as the vials are tightly sealed to prevent desiccation of the sample. Therefore, many times venous blood is used for acid base status assessment although the composition of venous blood is affected by the. Blood sample collection nutrition centres this document is designed for online viewing. Compliance of blood sampling procedures with the clsi h3a6 guidelines. Frequency of preanalytical procedures in all wards in two district. Confirm the ordered tests and fill out the appropriate forms and labels.
In this sense, the sample is the gathered matter, and the sampling tool or sampler is the person or material to collect the sample. Your doctor may order this test to help diagnose diabetes. Neonatal pain response and adverse effects and maternal anxiety were assessed in 27 infants who were randomly allocated to venepuncture or heel stick. Turbid samples or those with particulate matter should be centrifuged prior to assay. Adults blood sampling in amounts exceeding 200 cc, drawn at one time. Hemodraw closed blood sampling system, pressure monitoring.
Private hospitals, day procedure centres and mobile health services in victoria must be registered and comply with regulations on patient safety and care boards and governance victorian health service boards have well defined responsibilities and the department acknowledges board education as a. Sterile gauze padsfor removal of first freeflowing drop of blood and for. Patients in hospitals are provided with identification bracelets which display a patient identity number in a machinereadable form, such as a bar code. A procedure for arterial blood sampling in the rabbit article pdf available in laboratory animals 202. Who guidelines on drawing blood best practices in phlebotomy eng. New microfluidicbased sampling procedure for overcoming the. Standard operating procedure collection of venous blood. To avoid penetration of the calcaneus and the risk for osteochondritis, puncture only the most medial and lateral portions of the plantar surface of the heel. For blood lead tests vigorous agitation is not an issue because it is more important to prevent clotting than cell rupture. Small amount of blood is drawn from arteries, which can be, radial artery, femoral artery, brachial artery or another suitable one. Pediatric vascular access and blood sampling techniques. Nips scores were higher in the heel stick group compared with the venepuncture group. Collecting, preserving and shipping specimens for the.
Genieur standard operating procedure sop blood sampling and transportation personnel appropriate staff to undertake venepuncture may include. Collection is by a closed method, either a hypodermic syringe or a vacuumized container. The rationale of this standard operating procedure is as follows. If the sample data will be generalized to reflect the population, statistical selection processes should be used, e. What precautions should be taken, while collecting sample. Mar 15, 2017 blood clotting time determination by capillary method.
Raj, p10 such samples are usually selected with the help of random numbers. Phlebotomy the drawing of blood has been practised for centuries and is still one of the most. Raj, p4 the surveyors a person or a establishment in charge of collecting and recording data or researchers initial task is to formulate a. Minute quantities of blood can be taken from various sites by pricking the skin. Sampling equipment needs to be chosen based on the analysis being performed as well as the consistency of the material being sampled. How to safely collect blood samples from persons suspected to be infected with highly infectious blood borne pathogens e. It offers guidance on the requirements for ensuring that blood collection is a safe and patientcentred procedure.
A procedure where both the person administering the treatment and the participant dont know which treatment the participant is receiving helps minimize experimenter bias another member of the research team keeps track of which condition each participant has been assigned to the doubleblind procedure also helps researchers. Any documents appearing in paper form are not controlled and should be checked against the electronic file version prior to use. Composite sampling is performed when a sample is taken over a specific amount of time or a specific amount of flow. Rare validation of the sampling sites often leads to erroneous estimation of pk parameters. The validity of test results can be voided if any of the following occur.